9E6A
Pseudomonas putida KT2440 IclR-type transcription factor (PP_2609)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-08-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.598, 69.440, 95.684 |
| Unit cell angles | 90.00, 101.99, 90.00 |
Refinement procedure
| Resolution | 38.090 - 2.110 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.911 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.500 | 2.115 |
| High resolution limit [Å] | 2.100 | 2.108 |
| Rmerge | 0.110 | 0.461 |
| Rmeas | 0.132 | 0.554 |
| Rpim | 0.071 | 0.305 |
| Number of reflections | 66102 | 680 |
| <I/σ(I)> | 7.2 | 2.3 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 3.4 | 3.2 |
| CC(1/2) | 0.986 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M Tris-HCl pH 8, 0.2M Li2SO4, 0.02M Ammonium Acetate, 8% w/v PEG3350, Arabidopsis thaliana root exudates |
