9E4W
Structure of Bacillus phage SPO1 anti-CBASS 4 (Acb4) in complex with 3'3'-cGAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2024-02-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.284, 56.496, 167.581 |
| Unit cell angles | 87.53, 89.02, 81.58 |
Refinement procedure
| Resolution | 45.600 - 2.080 |
| R-factor | 0.2194 |
| Rwork | 0.219 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.403 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.710 | 2.260 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rpim | 0.092 | 0.388 |
| Number of reflections | 82527 | 4126 |
| <I/σ(I)> | 4.2 | 1.5 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.988 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 18% PEG-4000, Tris-HCl pH 8.80, and 0.1 M MgCl2 |
