9E4H
Human ASIC1a at pH 5.7 with rotated, domain-swapped transmembrane domain
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 0.01 |
| Detector | GATAN K3 BIOCONTINUUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.61 |
| Software | cryoSPARC,PHENIX,SerialEM,Coot,ISOLDE |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
