9E48
Crystal Structure of FosA from Pseudomonas aeruginosa with Manganese and Fosfomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU PhotonJet-R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5406 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.784, 67.208, 76.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.290 - 1.800 |
| R-factor | 0.1685 |
| Rwork | 0.166 |
| R-free | 0.19380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.762 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.290 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.027 | 0.090 |
| Number of reflections | 26765 | 1794 |
| <I/σ(I)> | 21.5 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 85 mM K2HPO4 (pH 7.0), 41.5% pentaerythritol propoxylate 17/8 PO/OH (PEP629), 1mM MnCl2, 40 mM KCl |
