Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.544, 81.654, 89.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.310 - 1.800 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.18010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.108 | 0.060 | 0.263 |
| Rmeas | 0.115 | 0.063 | 0.287 |
| Rpim | 0.037 | 0.019 | 0.112 |
| Total number of observations | 230090 | ||
| Number of reflections | 25633 | 1425 | 1273 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 99.8 | 99.6 | 99.7 |
| Redundancy | 9 | 10.7 | 6.4 |
| CC(1/2) | 0.993 | 0.998 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | Tris pH 7.0, 40% PEG 300, and 5% PEG 1000 |
