9E0S
Cryo-EM structure of a the periplasmic insert from Myxococcus TAtC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 4 2 2 |
| Unit cell lengths | 110.776, 110.776, 101.898 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.100 - 1.980 |
| R-factor | 0.1892 |
| Rwork | 0.187 |
| R-free | 0.22350 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.740 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (dev_5278) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.100 | 2.030 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rpim | 0.054 | |
| Number of reflections | 44477 | 3065 |
| <I/σ(I)> | 11.7 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.2 | 25.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 296 | lithium sulphate, Tris pH 8.5, PEG 8000, PEG 1000 |
