9DYT
Acanthamoeba Polyphaga Mimivirus R699
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2022-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.821, 121.696, 72.690 |
| Unit cell angles | 90.00, 118.59, 90.00 |
Refinement procedure
| Resolution | 61.383 - 1.800 |
| Rwork | 0.190 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.536 |
| Data reduction software | xia2 (3.8.0) |
| Data scaling software | Aimless (0.7.8) |
| Phasing software | PHENIX (1.20.1) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.383 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.079 | 0.908 |
| Number of reflections | 97211 | 4789 |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 6.8 | 6.9 |
| CC(1/2) | 0.998 | 0.864 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | Protein/cofactor mix: 30mg/ml R699, 2mM MnCl2. mother liquor: 0.2 M Ammonium formate, 20% (w/v) PEG 3350 The ratio of mix and mother liquor was at 1:1. |
