9DYB

CHIP-TPR in complex with the phosphorylated Hsp70 tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000000
Spacegroup name	P 2 21 21
Unit cell lengths	37.575, 45.932, 78.149
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.600 - 1.600
R-factor	0.1837
Rwork	0.180
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.782
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5127)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.600	1.640
High resolution limit [Å]	1.590	1.590
Rmerge	0.092	0.860
Rpim	0.055	0.532
Number of reflections	34506	8914
<I/σ(I)>	10.32	1.25
Completeness [%]	99.7	97.38
Redundancy	3.7	3.5
CC(1/2)	0.997	0.612

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.2 M lithium sulfate, 0.1 M Tris-HCl, pH 8.5, 30% (w/v) PEG 4000, 10 mM zinc chloride