9DXI

Crystal structure of dimer Caenorhabditis elegans lipid binding protein 3 (LBP-3)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	45.731, 68.846, 103.891
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.090 - 2.170
R-factor	0.2219
Rwork	0.219
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.260
High resolution limit [Å]	2.170	4.700	2.170
Rmerge	0.201	0.102	1.310
Rmeas	0.212	0.109	1.417
Rpim	0.065	0.038	0.528
Number of reflections	17848	1929	1705
<I/σ(I)>	5.4
Completeness [%]	99.7	99.1	98.9
Redundancy	10.1	8.7	6.7
CC(1/2)	0.998	0.996	0.634

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	14% PEG 3350, 0.16 M NaCl, 0.1 M Sodium Citrate