9DWS

X-ray crystal structure of Francisella hispaniensis apo ribonucleotide reductase beta subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	81.670, 98.030, 100.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.050 - 2.300
R-factor	0.1729
Rwork	0.171
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.786
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.050	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.150	1.720
Rmeas	0.155	0.155
Rpim	0.040	0.456
Number of reflections	36537	3612
<I/σ(I)>	13.78	2.08
Completeness [%]	99.9	99.97
Redundancy	14.8	14.9
CC(1/2)	0.998	0.705

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	8.5% (w/v) PEG 4000, 0.8 M lithium chloride, and 0.1 M Tris pH 8.5