9DVE
X-ray crystal structure of Kohinoor reversibly switchable fluorescent protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | .9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 138.530, 73.628, 93.219 |
| Unit cell angles | 90.00, 115.86, 90.00 |
Refinement procedure
| Resolution | 83.890 - 2.150 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.104 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.890 | 2.210 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.072 | 0.697 |
| Rmeas | 0.088 | 0.857 |
| Rpim | 0.050 | 0.492 |
| Total number of observations | 128853 | 9850 |
| Number of reflections | 44302 | 3406 |
| <I/σ(I)> | 8 | 1.5 |
| Completeness [%] | 96.0 | |
| Redundancy | 2.9 | 2.9 |
| CC(1/2) | 0.995 | 0.796 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | .12-.14M magnesium nitrate hexahydrate 18-24% PEG-3350 |
