9DUV
Cryo-EM structure of recombinant R254H ACTA1 phalloidin-stabilized F-actin
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS GLACIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.3 |
| Software | cryoSPARC,PHENIX,EPU,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
