Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.026, 205.045, 295.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.050 - 3.110 |
| R-factor | 0.2216 |
| Rwork | 0.221 |
| R-free | 0.25750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.434 |
| Data reduction software | XDS (20230630) |
| Data scaling software | XDS (20230630) |
| Phasing software | PHENIX (1.21.2_5419) |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.050 | 20.050 | 3.220 |
| High resolution limit [Å] | 3.110 | 6.630 | 3.110 |
| Rmerge | 0.260 | 0.075 | 1.354 |
| Number of reflections | 112050 | 12094 | 11431 |
| <I/σ(I)> | 4.9 | 12.5 | 1 |
| Completeness [%] | 96.0 | 99.9 | 99.02 |
| Redundancy | 4.4 | 4.34 | 4.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 6 mg/mL protein, 0.2% Triton X-100, 0.066 M Imidazole, 2 mM Magnesium Chloride, 8.75% w/v PEG1000, 8.75% w/v PEG3350, 8.75% v/v MPD |
