9DTL

Crystal Structure of C4-Dicarboxylate-Binding Protein (PA0884) of Tripartite ATP-independent Periplasmic Transporter Family from Pseudomonas aeruginosa PAO1 in Complex with Succinic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	46.010, 67.095, 86.653
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.550 - 1.300
R-factor	0.17314
Rwork	0.172
R-free	0.19533
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.331
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.092
Rmeas	0.099
Rpim	0.037	0.502
Number of reflections	65543	3205
<I/σ(I)>	25	1.9
Completeness [%]	98.4	97
Redundancy	7.2	7
CC(1/2)	0.993	0.661

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	Protein: 8.4 mg/ml, 0.15M Sodium chloride, 0.02M Tris-HCl (pH 8.0), 2mM Succinic acid; Screen: Classics II (A4), 0.1M SPG buffer pH 7, 25% (w/v) PEG 1500; Cryo: Reservoir.