9DTB

Crystal structure of the WDR domain of WDR91 in complex with DR3460

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2024-07-09
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	78.834, 132.976, 119.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.840 - 2.310
R-factor	0.19993
Rwork	0.198
R-free	0.24249
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.225
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.350
High resolution limit [Å]	2.310	6.270	2.310
Rmerge	0.058	0.023	0.981
Rmeas	0.062	0.025	1.049
Rpim	0.022	0.009	0.369
Total number of observations	222747
Number of reflections	27927	1493	1374
<I/σ(I)>	11.6
Completeness [%]	99.9	98	100
Redundancy	8	7.3	7.9
CC(1/2)	1.000	1.000	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	12.5%(w/v) PEG 4K, 20%(v/v) 1,2,6-hexanetriol 0.02 M of each Monosaccharide II 0.1 M MOPSO/bis-tris pH 6.5