9DT4
Crystal structure of the engineered sulfonylurea repressor CsR (L4.2-20), apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-11 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.120, 56.840, 106.007 |
| Unit cell angles | 90.00, 90.32, 90.00 |
Refinement procedure
| Resolution | 19.872 - 2.400 |
| Rwork | 0.178 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.788 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.872 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 16516 | 784 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.0 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M Sodium acetate 0.1 M TRIS hydrochloride pH 8.5 30% w/v Polyethylene glycol 4,000 |
