9DSY

Crystal Structure of C4-Dicarboxylate-Binding Periplasmic Protein (PA5167) of Tripartite ATP-independent Periplasmic Transporter Family from Pseudomonas aeruginosa PAO1 in Complex with Succinic Acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1
Unit cell lengths	34.585, 44.495, 47.239
Unit cell angles	78.78, 84.22, 75.83

Refinement procedure

Resolution	29.850 - 1.350
R-factor	0.17365
Rwork	0.173
R-free	0.18922
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.461
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.370
High resolution limit [Å]	1.350	1.350
Rmerge	0.103
Rmeas	0.121
Rpim	0.063	0.894
Number of reflections	55717	2681
<I/σ(I)>	13.4	2
Completeness [%]	95.4	92.9
Redundancy	3.7	4
CC(1/2)	0.987	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.3	292	Protein: 7.0 mg/ml, 0.5M Sodium chloride, 0.02M Tris-HCl (pH 8.0), 2mM Succinic acid; Screen: Classics II (H9), 0.05M Zing acetate, 20% (w/v) PEG 3350; Cryo: Reservoir.