9DR9
Binary product complex of DNA polymerase iota with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-11 |
Detector | NOIR-1 |
Wavelength(s) | 1 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 97.100, 97.100, 202.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.760 - 2.210 |
R-factor | 0.257 |
Rwork | 0.255 |
R-free | 0.29720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.017 |
RMSD bond angle | 1.834 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (1.20.1_4487) |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.550 | 2.290 |
High resolution limit [Å] | 2.060 | 2.210 |
Rpim | 0.018 | 0.419 |
Number of reflections | 28209 | 2057 |
<I/σ(I)> | 25.8 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 18.9 | 11.6 |
CC(1/2) | 1.000 | 0.766 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 291 | 100 mM HEPES pH 7 1.5 - 1.8 M Ammonium Sulfate 5 mM EDTA |