9DR7

Product complex of DNA polymerase iota with 2 monophosphates

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2019-03-08
Detector	NOIR-1
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	98.190, 98.190, 202.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.090 - 2.450
R-factor	0.2566
Rwork	0.254
R-free	0.31190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.609
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	2.560
High resolution limit [Å]	2.450	2.450
Rpim	0.049	0.469
Number of reflections	21926	2657
<I/σ(I)>	13.2	1.6
Completeness [%]	100.0	100
Redundancy	20	20
CC(1/2)	0.998	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291	100 mM HEPES pH 7 1.5 -1.8 M Ammonium Sulfate 5 mM EDTA