9DQQ
Crystal structure of HrmJ from Streptomyces sp. Ag109_G2-6 (HrmJ-ssa) complexed with ferric iron(III) and 2-oxoglutarate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-06 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 96.871, 43.616, 126.201 |
| Unit cell angles | 90.00, 106.95, 90.00 |
Refinement procedure
| Resolution | 17.680 - 2.450 |
| R-factor | 0.211 |
| Rwork | 0.207 |
| R-free | 0.27960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.883 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.680 | 2.550 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.104 | |
| Number of reflections | 18054 | 2016 |
| <I/σ(I)> | 11.9 | 1.9 |
| Completeness [%] | 96.0 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.996 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.22 M KSCN, 26% (w/v) PEG 3350 |
