9DQP

Crystal structure of apo-HrmJ from Streptomyces sp. Ag109_G2-6 (HrmJ-ssa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97946
Spacegroup name	C 1 2 1
Unit cell lengths	97.052, 42.931, 126.758
Unit cell angles	90.00, 107.03, 90.00

Refinement procedure

Resolution	38.960 - 1.950
R-factor	0.2007
Rwork	0.198
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.21)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge	0.084
Number of reflections	35459	1751
<I/σ(I)>	15.4	2.3
Completeness [%]	96.8	98.2
Redundancy	6.1
CC(1/2)	0.991	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.176 M KSCN, 20.8% (w/v) PEG 3350, 20 mM KCl