9DPV
BMP-9 K357R G389S Dimer Without Radiation Damage in Neutral pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 71.105, 71.105, 145.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.550 - 1.990 |
| R-factor | 0.2259 |
| Rwork | 0.224 |
| R-free | 0.26490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.248 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.370 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.146 | 1.467 |
| Rmeas | 0.168 | 1.723 |
| Rpim | 0.081 | 0.877 |
| Number of reflections | 13055 | 832 |
| <I/σ(I)> | 6.4 | 1.2 |
| Completeness [%] | 98.8 | 86.7 |
| Redundancy | 7 | 5.6 |
| CC(1/2) | 0.988 | 0.645 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289.15 | 1M NaCl, 22-26% glycerol, 0.1 M HEPES pH 7.5 |
