9DPR
BMP-9 Wild-Type Dimer with Radiation Damage in Acidic pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 71.044, 71.044, 145.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.340 - 2.610 |
R-factor | 0.2153 |
Rwork | 0.212 |
R-free | 0.26620 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.007 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.340 | 2.730 |
High resolution limit [Å] | 2.610 | 2.610 |
Rmerge | 0.301 | 2.521 |
Rmeas | 0.357 | 3.017 |
Rpim | 0.188 | 1.610 |
Number of reflections | 5804 | 684 |
<I/σ(I)> | 4.9 | 1.7 |
Completeness [%] | 98.0 | 97.9 |
Redundancy | 5.4 | 5.5 |
CC(1/2) | 0.953 | 0.265 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 289.15 | 1M NaCl, 3.5% PEG8K, 0.3M Sodium Citrate pH 3.5 |