9DPQ
BMP-9 Wild-Type Dimer without Radiation Damage in Acidic pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 71.045, 71.045, 145.567 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.340 - 2.350 |
| R-factor | 0.2027 |
| Rwork | 0.200 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.823 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.340 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.209 | 1.478 |
| Rmeas | 0.251 | 1.785 |
| Rpim | 0.135 | 0.973 |
| Number of reflections | 7868 | 763 |
| <I/σ(I)> | 4.8 | 1.7 |
| Completeness [%] | 97.9 | 98.3 |
| Redundancy | 5 | 4.6 |
| CC(1/2) | 0.982 | 0.311 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 289.15 | 1M NaCl, 3.5% PEG8K, 0.3M Sodium Citrate pH 3.5 |
