9DPM

BMP-9 Monomer Growth Factor with Cysteinylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-17
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9798
Spacegroup name	I 41 2 2
Unit cell lengths	71.863, 71.863, 144.606
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.460 - 1.900
R-factor	0.2064
Rwork	0.205
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.770
Data reduction software	HKL-3000
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.136	3.025
Rmeas	0.139	3.093
Rpim	0.028	0.643
Number of reflections	15387	962
<I/σ(I)>	11.6	0.7
Completeness [%]	100.0	99.9
Redundancy	23.78
CC(1/2)	0.998	0.598

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289.15	0.9 M sodium chloride, 133 mM HEPES, 166 mM MES