9DP8
A DARPin fused to the 1TEL crystallization chaperone via a helical Lys-Gln-Arg linker
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 65 |
| Unit cell lengths | 105.981, 105.981, 51.137 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.890 - 3.470 |
| R-factor | 0.272 |
| Rwork | 0.271 |
| R-free | 0.29790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.639 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.890 | 3.598 |
| High resolution limit [Å] | 3.470 | 3.474 |
| Rmerge | 0.127 | 3.200 |
| Rmeas | 0.134 | 3.390 |
| Rpim | 0.043 | 1.111 |
| Number of reflections | 3961 | 320 |
| <I/σ(I)> | 7.03 | 0.5 |
| Completeness [%] | 87.5 | 75.29 |
| Redundancy | 9.4 | 9.2 |
| CC(1/2) | 0.999 | 0.578 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 298 | 0.1 M BIS-TRIS pH 5.5, 0.3 M Magnesium formate dihydrate |
