9DP3
APE1 N174D Product Complex with Abasic DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-10 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.205, 60.834, 73.619 |
| Unit cell angles | 82.55, 77.17, 85.85 |
Refinement procedure
| Resolution | 24.950 - 2.100 |
| R-factor | 0.2546 |
| Rwork | 0.253 |
| R-free | 0.27440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-3000 (705c) |
| Data scaling software | HKL-3000 (705c) |
| Phasing software | PHENIX (1.19.2-4158-000) |
| Refinement software | PHENIX (1.19.2-4158-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.950 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.145 | |
| Number of reflections | 35000 | 1966 |
| <I/σ(I)> | 7.52 | 0.56 |
| Completeness [%] | 98.2 | 91.2 |
| Redundancy | 3.3 | 1.8 |
| CC(1/2) | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 200 mM lithium sulfate, 15-25% PEG3350 |
