9DP2
APE1 N174A Product Complex with Abasic DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-13 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.256, 61.831, 72.617 |
| Unit cell angles | 82.99, 78.28, 87.17 |
Refinement procedure
| Resolution | 34.030 - 1.990 |
| R-factor | 0.1859 |
| Rwork | 0.185 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.390 |
| Data reduction software | HKL-3000 (705c) |
| Data scaling software | HKL-3000 (705c) |
| Phasing software | PHENIX (1.19.2-4158-000) |
| Refinement software | PHENIX (1.19.2-4158-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.030 | 2.020 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmeas | 0.053 | |
| Number of reflections | 76015 | 2534 |
| <I/σ(I)> | 27.32 | 4.45 |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 3.9 | 3.6 |
| CC(1/2) | 0.934 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM sodium citrate, pH 5.0, 200 mM magnesium chloride, 16-21% PEG20000 |
