9DNT
Cryo-EM structure of Tom1 (S. cerevisiae)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3 |
| Number of Particle | 4216890 |
| Software | cryoSPARC,PHENIX,SerialEM,UCSF ChimeraX,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
