Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.92010 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 146.309, 146.309, 146.309 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.490 - 2.130 |
| R-factor | 0.2104 |
| Rwork | 0.210 |
| R-free | 0.21410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.093 |
| Data reduction software | AutoProcess |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.490 | 2.204 |
| High resolution limit [Å] | 2.130 | 2.135 |
| Rmerge | 0.104 | 1.567 |
| Rmeas | 0.110 | 1.639 |
| Rpim | 0.479 | |
| Number of reflections | 27481 | 1377 |
| <I/σ(I)> | 12.5 | 1.6 |
| Completeness [%] | 94.6 | |
| Redundancy | 10.4 | |
| CC(1/2) | 0.998 | 0.646 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES, pH 6.5, 1.0 M sodium citrate, 5.0 mM TCEP |
