9DJS

T4 Lysozyme A73H/G77H/T115H/R119H co-crystallized with Cu(II)-NTA (room temperature structure)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2024-08-22
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	60.721, 60.721, 97.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.610 - 1.550
R-factor	0.1769
Rwork	0.175
R-free	0.19680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.400
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.610	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	30207	2908
<I/σ(I)>	10.81	1.28
Completeness [%]	98.0	94.4
Redundancy	4.4	3.2
CC(1/2)	0.995	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	293	Protein: 0.33 mM T4 lysozyme mutant, 4.95 mM Cu(II)-NTA. Precipitant: 2.2 M NaH2PO4/K2HPO4, pH 6.6, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol