9DJP

T4 Lysozyme A73H/G77H/R119H/Q123H co-crystallized with Cu(II)-NTA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	59.514, 59.514, 96.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.420 - 1.170
R-factor	0.1454
Rwork	0.145
R-free	0.16070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.420	1.210
High resolution limit [Å]	1.170	1.170
Number of reflections	66047	4603
<I/σ(I)>	19.8	2.9
Completeness [%]	99.6	95
Redundancy	10.8	9.8
CC(1/2)	1.000	0.878

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Protein: 0.33 mM T4 lysozyme mutant, 2.64 mM Cu(II)-NTA. Precipitant: 2.2 M NaH2PO4/K2HPO4, pH 6.6, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol