9DJM

T4 Lysozyme K147H/T151H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	60.221, 60.221, 96.954
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.760 - 1.080
R-factor	0.1502
Rwork	0.150
R-free	0.16670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.760	1.110
High resolution limit [Å]	1.080	1.080
Number of reflections	86475	5709
<I/σ(I)>	19.9	1.8
Completeness [%]	99.0	89.3
Redundancy	9.8	4.8
CC(1/2)	1.000	0.777

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	Protein: 0.33 mM T4 lysozyme mutant. Precipitant: 2.0 M NaH2PO4/K2HPO4, pH 6.8, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol