9DJH
T4 Lysozyme T115H/R119H co-crystallized with Cu(II)-NTA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-09-28 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.099, 60.099, 97.502 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.580 - 1.450 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.121 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.580 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 35429 | 1189 |
| <I/σ(I)> | 35 | 2.8 |
| Completeness [%] | 95.5 | 63.6 |
| Redundancy | 10.6 | 3.5 |
| CC(1/2) | 1.000 | 0.766 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | Protein: 0.33 mM T4 lysozyme mutant, 2.475 mM Cu(II)-NTA. Precipitant: 2.2 M NaH2PO4/K2HPO4, pH 6.8, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol |
