9DJB

T4 Lysozyme A73H/G77H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 32 2 1
Unit cell lengths	59.859, 59.859, 95.823
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.190 - 1.130
R-factor	0.1586
Rwork	0.158
R-free	0.16950
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.928
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.570	1.160
High resolution limit [Å]	1.130	1.130
Number of reflections	74892	5298
<I/σ(I)>	14.6	3.2
Completeness [%]	99.8	97.7
Redundancy	10.9	10.2
CC(1/2)	0.999	0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8	293	Protein: 0.33 mM T4 lysozyme mutant Precipitant: 2.2 M NaH2PO4/K2HPO4, pH 6.8, 150 mM NaCl, 100 mM 1,6-hexanediol, 3% 2-propanol