9DHC
The Retinoblastoma Protein with Mutation S751Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 |
| Unit cell lengths | 255.036, 255.036, 35.358 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.200 - 2.320 |
| R-factor | 0.2153 |
| Rwork | 0.213 |
| R-free | 0.26280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.495 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.200 | 2.410 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 37129 | 3923 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10 |
| CC(1/2) | 0.998 | 0.302 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 18% PEG 8K, 0.1M sodium citrate, 0.1M succinate pH5.5, 1M lithium chloride |
