9DH2
Structure of Fab in complex with NKG2D extracellular domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2021-10-05 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.062, 224.433, 136.297 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 136.640 - 2.980 |
| R-factor | 0.19931 |
| Rwork | 0.197 |
| R-free | 0.24308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.716 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 136.640 | 3.030 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Rmerge | 0.097 | 0.401 |
| Rpim | 0.063 | 0.221 |
| Number of reflections | 56052 | 8586 |
| <I/σ(I)> | 6.71 | |
| Completeness [%] | 97.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.992 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.2 M ammonium tartrate dibasic, 20% w/v PEG 3,350 |
