9DGN
T-junction triangle 7-11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU R-AXIS IV |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-08-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 167.670, 167.670, 82.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.230 - 7.320 |
| R-factor | 0.1512 |
| Rwork | 0.143 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.388 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.550 |
| High resolution limit [Å] | 7.290 | 7.290 |
| Rpim | 0.063 | |
| Number of reflections | 1183 | 116 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.7 | |
| CC(1/2) | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | DNA strands mixed with TAE/Mg2+ and 0.001 M Magnesium chloride hexahydrate, 0.005 M Tris hydrochloride, pH=7.5, 0.16 M Ammonium sulfate, 0.23 M sucrose and crystallized against (NH4)2SO4 |
