9DFB
Q108K:K40L:T51V:T53C:R58A:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V in the dark at pH 3.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.964, 35.924, 63.938 |
| Unit cell angles | 85.70, 86.87, 65.22 |
Refinement procedure
| Resolution | 32.560 - 1.705 |
| R-factor | 0.2228 |
| Rwork | 0.220 |
| R-free | 0.25150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.047 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.560 | 1.765 |
| High resolution limit [Å] | 1.705 | 1.705 |
| Rmeas | 0.095 | |
| Rpim | 0.053 | 0.369 |
| Number of reflections | 20907 | 2010 |
| <I/σ(I)> | 19.11 | |
| Completeness [%] | 76.8 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8 |
