9DFAExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 291 |
| Detector technology | PIXEL |
| Collection date | 2022-07-02 |
| Detector | DECTRIS PILATUS3 X 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.650, 93.700, 97.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.070 - 2.170 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | XSCALE (Jan 10, 2022) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 10.000 | 2.270 |
| High resolution limit [Å] | 2.170 | 10.000 | 2.170 |
| Rmerge | 0.107 | 0.050 | 2.646 |
| Rmeas | 0.113 | 0.054 | 2.806 |
| Rpim | 0.038 | 0.992 | |
| Number of reflections | 45418 | 541 | 5676 |
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 99.9 | 97.1 | 100 |
| Redundancy | 8.97 | 7.4824 | 9.111698 |
| CC(1/2) | 0.998 | 0.998 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 291 | HEPES 0.08M pH7, 2-propanol 8.5%, PEG 4000 5%, [7.7mg/ml protein] |
