9DF8

Q108K:K40L:T51V:T53C:R58A:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V and irradiated with UV light at pH 3.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.127
Spacegroup name	P 1
Unit cell lengths	31.037, 36.018, 64.069
Unit cell angles	85.88, 86.75, 65.28

Refinement procedure

Resolution	32.670 - 1.780
R-factor	0.1933
Rwork	0.190
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.095
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.670	1.843
High resolution limit [Å]	1.779	1.779
Rmeas	0.066	0.493
Rpim	0.035	0.277
Number of reflections	20482	1917
<I/σ(I)>	19.53	2
Completeness [%]	84.9
Redundancy	3.4
CC(1/2)		0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	25% PEG4000, 100 mM ammonium acetate, 100 mM sodium acetate, pH 4.0-4.8