9DEL
USP7 in complex with macrocycle MC03
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-16 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.913, 69.417, 76.408 |
| Unit cell angles | 90.00, 91.93, 90.00 |
Refinement procedure
| Resolution | 42.810 - 2.500 |
| R-factor | 0.2189 |
| Rwork | 0.216 |
| R-free | 0.27210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.452 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.871 | 2.586 |
| High resolution limit [Å] | 2.397 | 2.397 |
| Rmerge | 0.028 | 0.640 |
| Rmeas | 0.039 | 0.905 |
| Rpim | 0.028 | 0.639 |
| Number of reflections | 20952 | 1049 |
| <I/σ(I)> | 15.2 | 1.4 |
| Completeness [%] | 75.6 | 45.69 |
| Redundancy | 2.8 | 2.9 |
| CC(1/2) | 0.999 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 25 %w/v PEG 1500, 0.1 M MMT pH 7 |
