9DED
Designed protein n8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-20 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.556, 31.558, 40.794 |
| Unit cell angles | 90.00, 101.32, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.770 |
| R-factor | 0.1933 |
| Rwork | 0.188 |
| R-free | 0.23690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.798 |
| Data reduction software | XDS |
| Phasing software | PHENIX (1.21_5207) |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.810 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.090 | 0.831 |
| Rpim | 0.041 | 0.368 |
| Number of reflections | 9440 | 526 |
| <I/σ(I)> | 11.4 | 2.4 |
| Completeness [%] | 98.8 | 99.3 |
| Redundancy | 6.7 | 6.8 |
| CC(1/2) | 0.997 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350 |
