9DE8
Structure of full-length HIV TAR RNA soaked in CaCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 31.588, 47.266, 132.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.530 - 2.760 |
| R-factor | 0.2477 |
| Rwork | 0.245 |
| R-free | 0.29510 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.424 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.530 | 2.860 |
| High resolution limit [Å] | 2.760 | 2.761 |
| Number of reflections | 5512 | 529 |
| <I/σ(I)> | 13.82 | 1.62 |
| Completeness [%] | 99.2 | |
| Redundancy | 42.8 | |
| CC(1/2) | 0.999 | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 50 mM sodium cacodylate pH 6.5, 80 mM NaCl, 12 mM spermine and 30% v/v 2-methyl-2,4-pentanediol (MPD). Soaking with 100 mM CaCl2 for 2 hours at 20 degrees Celsius |
