9DE7

Structure of full-length HIV TAR RNA G16A/A17G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	132.538, 32.580, 46.211
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.135 - 2.250
R-factor	0.1962
Rwork	0.193
R-free	0.22180
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.135	2.331
High resolution limit [Å]	2.250	2.250
Number of reflections	70998	7124
<I/σ(I)>	7.98	1.57
Completeness [%]	99.6
Redundancy	7.1
CC(1/2)	0.991	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	50 mM sodium cacodylate pH 6.5, 80 mM NaCl, 12 mM spermine and 30% v/v 2-methyl-2,4-pentanediol (MPD)