9DE6

Structure of full-length HIV TAR RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	61.253, 41.982, 68.570
Unit cell angles	90.00, 93.44, 90.00

Refinement procedure

Resolution	35.790 - 2.350
R-factor	0.2652
Rwork	0.264
R-free	0.28250
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.790	2.434
High resolution limit [Å]	2.350	2.350
Number of reflections	17435	8976
<I/σ(I)>	14.38	1.63
Completeness [%]	97.5
Redundancy	6.6
CC(1/2)	0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	293	12 mM NaCl, 80 mM KCl, 50 mM sodium cacodylate pH 5.5, 2 mM hexamine cobalt and 45% v/v MPD