9DE5
Structure of full-length HIV TAR RNA bound to HIV Tat RNA-binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.872, 40.515, 120.406 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 47.090 - 2.750 |
| R-factor | 0.2504 |
| Rwork | 0.248 |
| R-free | 0.28790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.557 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.093 | 2.847 |
| High resolution limit [Å] | 2.749 | 2.749 |
| Number of reflections | 17526 | 1742 |
| <I/σ(I)> | 4.89 | 1.16 |
| Completeness [%] | 97.8 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.994 | 0.711 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 12 mM NaCl, 80 mM KCl, 50 mM sodium cacodylate pH 5.5, 2 mM hexamine cobalt and 45% v/v MPD |
