9DDY
The Crystal Structure of Geranyltranstransferase from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.643, 101.496, 119.889 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.320 |
| R-factor | 0.2113 |
| Rwork | 0.209 |
| R-free | 0.25360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.444 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Rmerge | 0.111 | 0.909 |
| Rmeas | 0.122 | 1.011 |
| Rpim | 0.049 | 0.436 |
| Number of reflections | 28000 | 1383 |
| <I/σ(I)> | 12.5 | 1.04 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6 | 5.2 |
| CC(1/2) | 0.997 | 0.570 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 286 | 0.2 M Sodium chloride, 0.1 M BIS-TRIS, 25%w/v Polyethylene glycol 3,350 |
