9DDU

Q108K:K40L:T51V:T53C:R58Y:Y19W:Q38L:Q4A:T29L mutant of hCRBPII bound to FR1V and irradiated with UV light at pH 3.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-27
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.272
Spacegroup name	P 1
Unit cell lengths	31.021, 35.939, 64.043
Unit cell angles	86.01, 86.51, 65.28

Refinement procedure

Resolution	29.630 - 1.599
Rwork	0.193
R-free	0.21790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.630	1.657
High resolution limit [Å]	1.599	1.599
Rmeas	0.097	0.447
Rpim	0.038	0.192
Number of reflections	29102	2868
<I/σ(I)>	88.1
Completeness [%]	87.8	85.63
Redundancy	6.5
CC(1/2)		0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, sodium acetate, pH 4.0-4.8