9DDT

AAGAB pseudoGTPase domain in complex with AP-1 clathrin adaptor complex sigma 3 subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	50.294, 61.608, 92.854
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.430 - 1.680
R-factor	0.1843
Rwork	0.182
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.430	1.740
High resolution limit [Å]	1.680	1.680
Rmerge	0.074	0.829
Rmeas	0.077
Rpim	0.021
Number of reflections	33173	3112
<I/σ(I)>	18.51
Completeness [%]	98.7
Redundancy	12.9
CC(1/2)	0.999	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.1M Bis-Tris pH 6.5, 25% PEG3350